FIBRINOGEN-SPECIFIC PROTEASE IN THE Vipera renardi SNAKE VENOM

Aim. To search fibrinogenolytic enzymes among protein components of Vipera renardi snake venom. Methods. Venom V. as the lyophilized powder was supplied by Trypillia serpentarium. It dissolved in 0.05 M Tris-HCl buffer pH 8.3 and fractionated on Superdex G-75 using FPLC system Acta Prime. Peaks were tested for their ability to directly cleave fibrinogen. Hydrolytic products analyzed SDS-PAGE. Enzyme-electrophoresis with fibrinogen co-polymerized 12% polyacrylamide gel used identification that can fibrinogen.. Results. 4 fractions size-exclusion chromatography. SDS-PAGE hydrolysis showed presence fibrinogen-specific protease 1st 2nd fraction much more active according data enzyme electrophoresis contained molecular mass 25 kDa. Conclusions. Fractionation venom allowed detect a apparent kDa molecule.